8BVF

MoeA2 from Corynebacterium glutamicum in complex with FtsZ-CTD

8BVF の概要

• エントリーDOI: 10.2210/pdb8bvf/pdb
• 関連するPDBエントリー: 8BVE
• 分子名称: Molybdopterin molybdenumtransferase, Cell division protein FtsZ, SODIUM ION, ... (5 entities in total)
• 機能のキーワード: molybdopterin molybdotransferase; cell division; gephyrin-like protein; corynebacteriales, cell cycle
• 由来する生物種: Corynebacterium glutamicum ATCC 13032; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 91570.57

構造登録者

Martinez, M.,Haouz, A.,Wehenkel, A.M.,Alzari, P.M. (登録日: 2022-12-03, 公開日: 2023-02-22, 最終更新日: 2024-10-23)

主引用文献

Martinez, M.,Petit, J.,Leyva, A.,Sogues, A.,Megrian, D.,Rodriguez, A.,Gaday, Q.,Ben Assaya, M.,Portela, M.M.,Haouz, A.,Ducret, A.,Grangeasse, C.,Alzari, P.M.,Duran, R.,Wehenkel, A.M.
Eukaryotic-like gephyrin and cognate membrane receptor coordinate corynebacterial cell division and polar elongation.
Nat Microbiol, 8:1896-1910, 2023

PubMed Abstract: The order Corynebacteriales includes major industrial and pathogenic Actinobacteria such as Corynebacterium glutamicum or Mycobacterium tuberculosis. These bacteria have multi-layered cell walls composed of the mycolyl-arabinogalactan-peptidoglycan complex and a polar growth mode, thus requiring tight coordination between the septal divisome, organized around the tubulin-like protein FtsZ, and the polar elongasome, assembled around the coiled-coil protein Wag31. Here, using C. glutamicum, we report the discovery of two divisome members: a gephyrin-like repurposed molybdotransferase (Glp) and its membrane receptor (GlpR). Our results show how cell cycle progression requires interplay between Glp/GlpR, FtsZ and Wag31, showcasing a crucial crosstalk between the divisome and elongasome machineries that might be targeted for anti-mycobacterial drug discovery. Further, our work reveals that Corynebacteriales have evolved a protein scaffold to control cell division and morphogenesis, similar to the gephyrin/GlyR system that mediates synaptic signalling in higher eukaryotes through network organization of membrane receptors and the microtubule cytoskeleton.

PubMed: 37679597
DOI: 10.1038/s41564-023-01473-0

実験手法

X-RAY DIFFRACTION (2.68 Å)