8BVF

MoeA2 from Corynebacterium glutamicum in complex with FtsZ-CTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 43 21 2
Unit cell lengths	95.580, 95.580, 229.522
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.200 - 2.680
R-factor	0.187
Rwork	0.184
R-free	0.24020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.948
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487-000)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.200	2.810
High resolution limit [Å]	2.680	2.680
Rpim	0.050	0.296
Number of reflections	30746	3905
<I/σ(I)>	14.6
Completeness [%]	99.6
Redundancy	9.6
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.1M NaCl, 0.1M Bis-Tris pH 6.5, 1.5M (NH4)2SO4