8BTT
Structure of human RTCB
Summary for 8BTT
| Entry DOI | 10.2210/pdb8btt/pdb |
| Related | 8BTX |
| Descriptor | RNA-splicing ligase RtcB homolog, MANGANESE (II) ION (3 entities in total) |
| Functional Keywords | human, ligase, trna splicing |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 2 |
| Total formula weight | 116882.89 |
| Authors | Kopp, J.,Gerber, J.L.,Peschek, J. (deposition date: 2022-11-30, release date: 2023-12-13, Last modification date: 2024-04-17) |
| Primary citation | Gerber, J.L.,Morales Guzman, S.I.,Worf, L.,Hubbe, P.,Kopp, J.,Peschek, J. Structural and mechanistic insights into activation of the human RNA ligase RTCB by Archease. Nat Commun, 15:2378-2378, 2024 Cited by PubMed Abstract: RNA ligases of the RTCB-type play an essential role in tRNA splicing, the unfolded protein response and RNA repair. RTCB is the catalytic subunit of the pentameric human tRNA ligase complex. RNA ligation by the tRNA ligase complex requires GTP-dependent activation of RTCB. This active site guanylylation reaction relies on the activation factor Archease. The mechanistic interplay between both proteins has remained unknown. Here, we report a biochemical and structural analysis of the human RTCB-Archease complex in the pre- and post-activation state. Archease reaches into the active site of RTCB and promotes the formation of a covalent RTCB-GMP intermediate through coordination of GTP and metal ions. During the activation reaction, Archease prevents futile RNA substrate binding to RTCB. Moreover, monomer structures of Archease and RTCB reveal additional states within the RNA ligation mechanism. Taken together, we present structural snapshots along the reaction cycle of the human tRNA ligase. PubMed: 38493148DOI: 10.1038/s41467-024-46568-2 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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