8BRS

Crystal structure of a variant of penicillin G acylase from Bacillaceae i. s. sp. FJAT-27231 with reduced surface entropy and additionally engineered crystal contact.

8BRS の概要

• エントリーDOI: 10.2210/pdb8brs/pdb
• 分子名称: Penicillin G acylase, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, (R,R)-2,3-BUTANEDIOL, ... (6 entities in total)
• 機能のキーワード: penicillin, acylase, hydrolase, surface entropy reduction, crystal contact engineering, crystallizability
• 由来する生物種: Bacillus sp. FJAT-27231; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 86659.49

構造登録者

Wichmann, J.,Mayer, J.,Mattes, H.,Lukat, P.,Blankenfeldt, W.,Biedendieck, R. (登録日: 2022-11-23, 公開日: 2023-05-03, 最終更新日: 2024-02-07)

• 主引用文献: Wichmann, J.,Mayer, J.,Hintmann, M.,Lukat, P.,Blankenfeldt, W.,Biedendieck, R.; Multistep Engineering of a Penicillin G Acylase for Systematic Improvement of Crystallization Efficiency; Cryst.Growth Des., 2023; DOI: 10.1021/acs.cgd.2c01408

実験手法

X-RAY DIFFRACTION (1.2 Å)