8BO9

NanoLuc-D9R/H57A/K89R mutant complexed with azacoelenterazine bound in intra-barrel catalytic site

Summary for 8BO9

Entry DOI	10.2210/pdb8bo9/pdb
Descriptor	Non structural polyprotein, 3-(4-hydroxyphenyl)-8-[(4-hydroxyphenyl)methyl]-5-(phenylmethyl)-1$l^{4},4,7,8-tetrazabicyclo[4.3.0]nona-1(6),2,4-trien-9-one (3 entities in total)
Functional Keywords	nanoluc-type luciferase with bound substrate analogue - azacoelenterazine, luminescent protein
Biological source	synthetic construct
Total number of polymer chains	3
Total formula weight	62453.25
Authors	Marek, M.,Janin, L.Y. (deposition date: 2022-11-15, release date: 2023-09-27, Last modification date: 2023-12-13)
Primary citation	Nemergut, M.,Pluskal, D.,Horackova, J.,Sustrova, T.,Tulis, J.,Barta, T.,Baatallah, R.,Gagnot, G.,Novakova, V.,Majerova, M.,Sedlackova, K.,Marques, S.M.,Toul, M.,Damborsky, J.,Prokop, Z.,Bednar, D.,Janin, Y.L.,Marek, M. Illuminating the mechanism and allosteric behavior of NanoLuc luciferase. Nat Commun, 14:7864-7864, 2023 Cited by PubMed: 38030625 DOI: 10.1038/s41467-023-43403-y PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (3.1 Å)

Structure validation