8BO9

NanoLuc-D9R/H57A/K89R mutant complexed with azacoelenterazine bound in intra-barrel catalytic site

8BO9 の概要

• エントリーDOI: 10.2210/pdb8bo9/pdb
• 分子名称: Non structural polyprotein, 3-(4-hydroxyphenyl)-8-[(4-hydroxyphenyl)methyl]-5-(phenylmethyl)-1$l^{4},4,7,8-tetrazabicyclo[4.3.0]nona-1(6),2,4-trien-9-one (3 entities in total)
• 機能のキーワード: nanoluc-type luciferase with bound substrate analogue - azacoelenterazine, luminescent protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 62453.25

構造登録者

Marek, M.,Janin, L.Y. (登録日: 2022-11-15, 公開日: 2023-09-27, 最終更新日: 2023-12-13)

• 主引用文献: Nemergut, M.,Pluskal, D.,Horackova, J.,Sustrova, T.,Tulis, J.,Barta, T.,Baatallah, R.,Gagnot, G.,Novakova, V.,Majerova, M.,Sedlackova, K.,Marques, S.M.,Toul, M.,Damborsky, J.,Prokop, Z.,Bednar, D.,Janin, Y.L.,Marek, M.; Illuminating the mechanism and allosteric behavior of NanoLuc luciferase.; Nat Commun, 14:7864-7864, 2023; PubMed: 38030625; DOI: 10.1038/s41467-023-43403-y

実験手法

X-RAY DIFFRACTION (3.1 Å)