8BKF

Structure of E. coli Class 2 L-asparaginase EcAIII, mutant M200T (crystal M200T#o)

これはPDB形式変換不可エントリーです。

8BKF の概要

• エントリーDOI: 10.2210/pdb8bkf/pdb
• 分子名称: Isoaspartyl peptidase subunit alpha, Isoaspartyl peptidase subunit beta, SODIUM ION, ... (6 entities in total)
• 機能のキーワード: l-asparaginase, isoaspartylpeptidase, mutation, ntn-hydrolase, hydrolase
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 67035.75

構造登録者

Sciuk, A.,Ruszkowski, M.,Jaskolski, M.,Loch, J.I. (登録日: 2022-11-09, 公開日: 2023-05-03, 最終更新日: 2024-02-07)

主引用文献

Janicki, M.,Sciuk, A.,Zielezinski, A.,Ruszkowski, M.,Ludwikow, A.,Karlowski, W.M.,Jaskolski, M.,Loch, J.I.
The effects of nature-inspired amino acid substitutions on structural and biochemical properties of the E. coli L-asparaginase EcAIII.
Protein Sci., 32:e4647-e4647, 2023

PubMed Abstract: The Escherichia coli enzyme EcAIII catalyzes the hydrolysis of L-Asn to L-Asp and ammonia. Using a nature-inspired mutagenesis approach, we designed and produced five new EcAIII variants (M200I, M200L, M200K, M200T, M200W). The modified proteins were characterized by spectroscopic and crystallographic methods. All new variants were enzymatically active, confirming that the applied mutagenesis procedure has been successful. The determined crystal structures revealed new conformational states of the EcAIII molecule carrying the M200W mutation and allowed a high-resolution observation of an acyl-enzyme intermediate with the M200L mutant. In addition, we performed structure prediction, substrate docking, and molecular dynamics simulations for 25 selected bacterial orthologs of EcAIII, to gain insights into how mutations at the M200 residue affect the active site and substrate binding mode. This comprehensive strategy, including both experimental and computational methods, can be used to guide further enzyme engineering and can be applied to the study of other proteins of medicinal or biotechnological importance.

PubMed: 37095066
DOI: 10.1002/pro.4647

実験手法

X-RAY DIFFRACTION (1.221 Å)