8BIT

Crystal structure of acyl-CoA synthetase from Metallosphaera sedula in complex with Coenzyme A and acetyl-AMP

Summary for 8BIT

• Entry DOI: 10.2210/pdb8bit/pdb
• Descriptor: 4-hydroxybutyrate--CoA ligase 1, [[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] ethanoate, COENZYME A (3 entities in total)
• Functional Keywords: ligase, coa-ligase, acyl-coa ligase, acetyl-coa ligase, thermostable ligase, thermostable, acetyl-amp, substrate bound, monomer
• Biological source: Metallosphaera sedula DSM 5348
• Total number of polymer chains: 2
• Total formula weight: 132762.76

Authors

Capra, N.,Thunnissen, A.M.W.H.,Janssen, D.B. (deposition date: 2022-11-02, release date: 2023-11-15, Last modification date: 2024-03-13)

• Primary citation: Capra, N.,Lelievre, C.,Toure, O.,Fossey-Jouenne, A.,Vergne-Vaxelaire, C.,Janssen, D.B.,Thunnissen, A.M.W.H.,Zaparucha, A.; Adapting an acyl CoA ligase from Metallosphaera sedula for lactam formation by structure-guided protein engineering; Front Catal, 4:-, 2024; DOI: 10.3389/fctls.2024.1360129

Experimental method

X-RAY DIFFRACTION (3.1 Å)