8BGM

Crystal structure of the OrfX1-OrfX3 complex from the PMP1 neurotoxin gene cluster

Summary for 8BGM

Entry DOI	10.2210/pdb8bgm/pdb
Descriptor	Toxin (2 entities in total)
Functional Keywords	pmp1 clostridial neurotoxins orfx gene cluster botulinum tulip, toxin
Biological source	Paraclostridium bifermentans More
Total number of polymer chains	4
Total formula weight	147197.39
Authors	Kosenina, S.,Stenmark, P. (deposition date: 2022-10-28, release date: 2022-12-07, Last modification date: 2024-05-01)
Primary citation	Kosenina, S.,Stenmark, P. Crystal structure of the OrfX1-OrfX3 complex from the PMP1 neurotoxin gene cluster. Febs Lett., 597:515-523, 2023 Cited by PubMed Abstract: Paraclostridial mosquitocidal protein 1 (PMP1) is a member of the clostridial neurotoxin (CNT) family, which includes botulinum and tetanus neurotoxins. PMP1 has unique selectivity for anopheline mosquitos and is the only known member of the family that targets insects. PMP1 is encoded in an orfX gene cluster, which in addition to the toxin, consists of OrfX1, OrfX2, OrfX3, P47 and NTNH, which have been shown to aid in PMP1 toxicity. We here show that OrfX1 and OrfX3 form a complex and present its structure at 2.7 Å. The OrfX1-OrfX3 complex mimics the structure of full-length OrfX2 and belongs to the lipid-binding TULIP protein superfamily. With this report, the structures of all proteins encoded in the orfX gene cluster of CNTs are now determined. PubMed: 36403098 DOI: 10.1002/1873-3468.14542 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.7 Å)

Structure validation