8BFI

human CNOT1-CNOT10-CNOT11 module

8BFI の概要

• エントリーDOI: 10.2210/pdb8bfi/pdb
• 分子名称: CCR4-NOT transcription complex subunit 1, CCR4-NOT transcription complex subunit 10, CCR4-NOT transcription complex subunit 11 (3 entities in total)
• 機能のキーワード: n-terminal module ccr4-not complex, rna binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 218299.65

構造登録者

Basquin, J.,Ozgur, S.,Conti, E. (登録日: 2022-10-26, 公開日: 2022-12-28, 最終更新日: 2023-02-15)

主引用文献

Mauxion, F.,Basquin, J.,Ozgur, S.,Rame, M.,Albrecht, J.,Schafer, I.,Seraphin, B.,Conti, E.
The human CNOT1-CNOT10-CNOT11 complex forms a structural platform for protein-protein interactions.
Cell Rep, 42:111902-111902, 2023

PubMed Abstract: The evolutionary conserved CCR4-NOT complex functions in the cytoplasm as the main mRNA deadenylase in both constitutive mRNA turnover and regulated mRNA decay pathways. The versatility of this complex is underpinned by its modular multi-subunit organization, with distinct structural modules actuating different functions. The structure and function of all modules are known, except for that of the N-terminal module. Using different structural approaches, we obtained high-resolution data revealing the architecture of the human N-terminal module composed of CNOT1, CNOT10, and CNOT11. The structure shows how two helical domains of CNOT1 sandwich CNOT10 and CNOT11, leaving the most conserved domain of CNOT11 protruding into solvent as an antenna. We discovered that GGNBP2, a protein identified as a tumor suppressor and spermatogenic factor, is a conserved interacting partner of the CNOT11 antenna domain. Structural and biochemical analyses thus pinpoint the N-terminal CNOT1-CNOT10-CNOT11 module as a conserved protein-protein interaction platform.

PubMed: 36586408
DOI: 10.1016/j.celrep.2022.111902

実験手法

X-RAY DIFFRACTION (3 Å)