8BBF

Structure of the IFT-A complex; IFT-A1 module

8BBF の概要

• エントリーDOI: 10.2210/pdb8bbf/pdb
• 関連するPDBエントリー: 8BBE 8BBG
• EMDBエントリー: 15954 15955
• 分子名称: WD repeat-containing protein 19, Intraflagellar transport protein 140 homolog, Intraflagellar transport protein 122 homolog (3 entities in total)
• 機能のキーワード: cilia, intraflagellar transport, membrane protein import, complex, transport protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 462975.34

構造登録者

Hesketh, S.J.,Mukhopadhyay, A.G.,Nakamura, D.,Toropova, K.,Roberts, A.J. (登録日: 2022-10-12, 公開日: 2022-12-07, 最終更新日: 2024-07-24)

主引用文献

Hesketh, S.J.,Mukhopadhyay, A.G.,Nakamura, D.,Toropova, K.,Roberts, A.J.
IFT-A structure reveals carriages for membrane protein transport into cilia.
Cell, 185:4971-, 2022

PubMed Abstract: Intraflagellar transport (IFT) trains are massive molecular machines that traffic proteins between cilia and the cell body. Each IFT train is a dynamic polymer of two large complexes (IFT-A and -B) and motor proteins, posing a formidable challenge to mechanistic understanding. Here, we reconstituted the complete human IFT-A complex and obtained its structure using cryo-EM. Combined with AlphaFold prediction and genome-editing studies, our results illuminate how IFT-A polymerizes, interacts with IFT-B, and uses an array of β-propeller and TPR domains to create "carriages" of the IFT train that engage TULP adaptor proteins. We show that IFT-A⋅TULP carriages are essential for cilia localization of diverse membrane proteins, as well as ICK-the key kinase regulating IFT train turnaround. These data establish a structural link between IFT-A's distinct functions, provide a blueprint for IFT-A in the train, and shed light on how IFT evolved from a proto-coatomer ancestor.

PubMed: 36462505
DOI: 10.1016/j.cell.2022.11.010

実験手法

ELECTRON MICROSCOPY (8 Å)