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8B7Y

Cryo-EM structure of the E.coli 70S ribosome in complex with the antibiotic Myxovalargin B.

This is a non-PDB format compatible entry.
Summary for 8B7Y
Entry DOI10.2210/pdb8b7y/pdb
Related7QQ3
EMDB information14121 15905
Related PRD IDPRD_002441
Descriptor23S ribosomal RNA, 50S ribosomal protein L16, 50S ribosomal protein L17, ... (57 entities in total)
Functional Keywordsribosome, antibiotic, myxovalargin b, myxb, fmet-trna, p-trna
Biological sourceEscherichia coli K-12
More
Total number of polymer chains52
Total formula weight2126168.93
Authors
Koller, T.O.,Graf, M.,Wilson, D.N. (deposition date: 2022-10-03, release date: 2023-01-25, Last modification date: 2023-02-01)
Primary citationKoller, T.O.,Scheid, U.,Kosel, T.,Herrmann, J.,Krug, D.,Boshoff, H.I.M.,Beckert, B.,Evans, J.C.,Schlemmer, J.,Sloan, B.,Weiner, D.M.,Via, L.E.,Moosa, A.,Ioerger, T.R.,Graf, M.,Zinshteyn, B.,Abdelshahid, M.,Nguyen, F.,Arenz, S.,Gille, F.,Siebke, M.,Seedorf, T.,Plettenburg, O.,Green, R.,Warnke, A.L.,Ullrich, J.,Warrass, R.,Barry 3rd, C.E.,Warner, D.F.,Mizrahi, V.,Kirschning, A.,Wilson, D.N.,Muller, R.
The Myxobacterial Antibiotic Myxovalargin: Biosynthesis, Structural Revision, Total Synthesis, and Molecular Characterization of Ribosomal Inhibition.
J.Am.Chem.Soc., 145:851-863, 2023
Cited by
PubMed Abstract: Resistance of bacterial pathogens against antibiotics is declared by WHO as a major global health threat. As novel antibacterial agents are urgently needed, we re-assessed the broad-spectrum myxobacterial antibiotic myxovalargin and found it to be extremely potent against . To ensure compound supply for further development, we studied myxovalargin biosynthesis in detail enabling production via fermentation of a native producer. Feeding experiments as well as functional genomics analysis suggested a structural revision, which was eventually corroborated by the development of a concise total synthesis. The ribosome was identified as the molecular target based on resistant mutant sequencing, and a cryo-EM structure revealed that myxovalargin binds within and completely occludes the exit tunnel, consistent with a mode of action to arrest translation during a late stage of translation initiation. These studies open avenues for structure-based scaffold improvement toward development as an antibacterial agent.
PubMed: 36603206
DOI: 10.1021/jacs.2c08816
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (3 Å)
Structure validation

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