8B7O

X-ray structure of Auranofin-human H-chain ferritin

これはPDB形式変換不可エントリーです。

8B7O の概要

• エントリーDOI: 10.2210/pdb8b7o/pdb
• 分子名称: Ferritin heavy chain, N-terminally processed, CHLORIDE ION, MAGNESIUM ION, ... (6 entities in total)
• 機能のキーワード: ferritin, gold, encapsulation, auranofin, anticancer, transport protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22629.35

構造登録者

Ferraro, G.,Merlino, A. (登録日: 2022-09-30, 公開日: 2022-12-28, 最終更新日: 2024-01-31)

主引用文献

Lucignano, R.,Pratesi, A.,Imbimbo, P.,Monti, D.M.,Picone, D.,Messori, L.,Ferraro, G.,Merlino, A.
Evaluation of Auranofin Loading within Ferritin Nanocages.
Int J Mol Sci, 23:-, 2022

PubMed Abstract: Auranofin (AF), a gold(I) compound that is currently used for the treatment of rheumatoid arthritis and is in clinical trials for its promising anticancer activity, was encapsulated within the human H-chain and the horse spleen ferritin nanocages using the alkaline disassembly/reassembly protocol. The aim of the work was to highlight possible differences in their drug loading capacity and efficacy. The drug-loaded ferritins were characterized via UV-vis absorption spectroscopy and inductively coupled plasma-atomic emission spectroscopy to assess AF encapsulation and to define the exact amount of gold atoms trapped in the Ft cavity. The crystal structures allowed us to define the nature of AF interaction with both ferritins and to identify the gold binding sites. Moreover, the biological characterization let us to obtain preliminary information on the cytotoxic effect of AF when bound to the human H-chain.

PubMed: 36430642
DOI: 10.3390/ijms232214162

実験手法

X-RAY DIFFRACTION (1.17 Å)