8B6U

Mpf2Ba1 monomer

Summary for 8B6U

• Entry DOI: 10.2210/pdb8b6u/pdb
• EMDB information: 15882 15883
• Descriptor: Mpf2Ba1, MAGNESIUM ION (3 entities in total)
• Functional Keywords: monomer, pore-forming protein, macpf, insecticidal, toxin
• Biological source: Pseudomonas monteilii
• Total number of polymer chains: 1
• Total formula weight: 53217.59

Authors

Marini, G.,Poland, B.,Leininger, C.,Lukoyanova, N.,Spielbauer, D.,Barry, J.,Altier, D.,Lum, A.,Scolaro, E.,Perez-Ortega, C.,Yalpani, N.,Sandahl, G.,Mabry, T.,Klever, J.,Nowatzki, T.,Zhao, J.Z.,Sethi, A.,Kassa, A.,Crane, V.,Lu, A.,Nelson, M.E.,Eswar, N.,Topf, M.,Saibil, H.R. (deposition date: 2022-09-27, release date: 2023-05-31, Last modification date: 2024-02-07)

Primary citation

Marini, G.,Poland, B.,Leininger, C.,Lukoyanova, N.,Spielbauer, D.,Barry, J.K.,Altier, D.,Lum, A.,Scolaro, E.,Ortega, C.P.,Yalpani, N.,Sandahl, G.,Mabry, T.,Klever, J.,Nowatzki, T.,Zhao, J.Z.,Sethi, A.,Kassa, A.,Crane, V.,Lu, A.L.,Nelson, M.E.,Eswar, N.,Topf, M.,Saibil, H.R.
Structural journey of an insecticidal protein against western corn rootworm.
Nat Commun, 14:4171-4171, 2023

PubMed Abstract: The broad adoption of transgenic crops has revolutionized agriculture. However, resistance to insecticidal proteins by agricultural pests poses a continuous challenge to maintaining crop productivity and new proteins are urgently needed to replace those utilized for existing transgenic traits. We identified an insecticidal membrane attack complex/perforin (MACPF) protein, Mpf2Ba1, with strong activity against the devastating coleopteran pest western corn rootworm (WCR) and a novel site of action. Using an integrative structural biology approach, we determined monomeric, pre-pore and pore structures, revealing changes between structural states at high resolution. We discovered an assembly inhibition mechanism, a molecular switch that activates pre-pore oligomerization upon gut fluid incubation and solved the highest resolution MACPF pore structure to-date. Our findings demonstrate not only the utility of Mpf2Ba1 in the development of biotechnology solutions for protecting maize from WCR to promote food security, but also uncover previously unknown mechanistic principles of bacterial MACPF assembly.

PubMed: 37443175
DOI: 10.1038/s41467-023-39891-7

Experimental method

X-RAY DIFFRACTION (2.134 Å)