8B5R

p97-p37-SPI substrate complex

Summary for 8B5R

• Entry DOI: 10.2210/pdb8b5r/pdb
• EMDB information: 15774 15778 15846 15847 15861
• Descriptor: Transitional endoplasmic reticulum ATPase, I3 sequence being threaded through the p97 channel, Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, ... (6 entities in total)
• Functional Keywords: aaa+ atpase, p97, vcp, cdc48, unfoldase, protein phosphatase-1, protein maturation, chaperone
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 11
• Total formula weight: 633013.85

Authors

van den Boom, J.,Marini, G.,Meyer, H.,Saibil, H. (deposition date: 2022-09-24, release date: 2023-06-07, Last modification date: 2024-07-24)

Primary citation

van den Boom, J.,Marini, G.,Meyer, H.,Saibil, H.R.
Structural basis of ubiquitin-independent PP1 complex disassembly by p97.
Embo J., 42:e113110-e113110, 2023

PubMed Abstract: The AAA+-ATPase p97 (also called VCP or Cdc48) unfolds proteins and disassembles protein complexes in numerous cellular processes, but how substrate complexes are loaded onto p97 and disassembled is unclear. Here, we present cryo-EM structures of p97 in the process of disassembling a protein phosphatase-1 (PP1) complex by extracting an inhibitory subunit from PP1. We show that PP1 and its partners SDS22 and inhibitor-3 (I3) are loaded tightly onto p97, surprisingly via a direct contact of SDS22 with the p97 N-domain. Loading is assisted by the p37 adapter that bridges two adjacent p97 N-domains underneath the substrate complex. A stretch of I3 is threaded into the central channel of the spiral-shaped p97 hexamer, while other elements of I3 are still attached to PP1. Thus, our data show how p97 arranges a protein complex between the p97 N-domain and central channel, suggesting a hold-and-extract mechanism for p97-mediated disassembly.

PubMed: 37264685
DOI: 10.15252/embj.2022113110

Experimental method

ELECTRON MICROSCOPY (6.1 Å)