8AI4

Crystal structure of radical SAM epimerase EpeE C223A mutant from Bacillus subtilis with [4Fe-4S] clusters, S-adenosyl-L-homocysteine and RiPP peptide 5 bound

Summary for 8AI4

Entry DOI	10.2210/pdb8ai4/pdb
Descriptor	Radical SAM core domain-containing protein, Putative exported peptide YydF, IRON/SULFUR CLUSTER, ... (8 entities in total)
Functional Keywords	radical sam, metalloenzyme, iron-sulfur, ripp, metal binding protein
Biological source	Bacillus subtilis More
Total number of polymer chains	4
Total formula weight	86601.44
Authors	Polsinelli, I.,Fyfe, C.D.,Legrand, P.,Kubiak, X.,Chavas, L.M.G.,Berteau, O.,Benjdia, A. (deposition date: 2022-07-25, release date: 2024-01-10, Last modification date: 2024-03-13)
Primary citation	Kubiak, X.,Polsinelli, I.,Chavas, L.M.G.,Fyfe, C.D.,Guillot, A.,Fradale, L.,Brewee, C.,Grimaldi, S.,Gerbaud, G.,Thureau, A.,Legrand, P.,Berteau, O.,Benjdia, A. Structural and mechanistic basis for RiPP epimerization by a radical SAM enzyme. Nat.Chem.Biol., 20:382-391, 2024 Cited by PubMed: 38158457 DOI: 10.1038/s41589-023-01493-1 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.75 Å)

Structure validation