8AF0

Crystal structure of human angiogenin and RNA duplex

8AF0 の概要

• エントリーDOI: 10.2210/pdb8af0/pdb
• 分子名称: RNA (5'-R(*GP*CP*CP*CP*GP*CP*CP*UP*GP*UP*CP*AP*CP*GP*CP*GP*GP*GP*C)-3'), Angiogenin, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: ribonuclease, rna duplex, rnase a-like, rna binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 40691.62

構造登録者

Sievers, K.,Ficner, R. (登録日: 2022-07-15, 公開日: 2022-09-14, 最終更新日: 2024-11-06)

主引用文献

Sievers, K.,Ficner, R.
Structure of angiogenin dimer bound to double-stranded RNA.
Acta Crystallogr.,Sect.F, 78:330-337, 2022

PubMed Abstract: Angiogenin is an unusual member of the RNase A family and is of great interest in multiple pathological contexts. Although it has been assigned various regulatory roles, its core catalytic function is that of an RNA endonuclease. However, its catalytic efficiency is comparatively low and this has been linked to a unique C-terminal helix which partially blocks its RNA-binding site. Assuming that binding to its RNA substrate could trigger a conformational rearrangement, much speculation has arisen on the topic of the interaction of angiogenin with RNA. To date, no structural data on angiogenin-RNA interactions have been available. Here, the structure of angiogenin bound to a double-stranded RNA duplex is reported. The RNA does not reach the active site of angiogenin and no structural arrangement of the C-terminal domain is observed. However, angiogenin forms a previously unobserved crystallographic dimer that makes several backbone interactions with the major and minor grooves of the RNA double helix.

PubMed: 36048083
DOI: 10.1107/S2053230X22008317

実験手法

X-RAY DIFFRACTION (2.43 Å)