8A8O
PAPS reductase from Methanothermococcus thermolithotrophicus refined to 1.45 A
Summary for 8A8O
Entry DOI | 10.2210/pdb8a8o/pdb |
Descriptor | Alpha-subunit of the PAPS reductase from Methanothermococcus thermolithotrophicus, Beta-subunit of the PAPS reductase from Methanothermococcus thermolithotrophicus, FLAVIN-ADENINE DINUCLEOTIDE, ... (10 entities in total) |
Functional Keywords | sulfate assimilation, methanogens, archaea, paps, pap, sulfite, thermophile, oxidoreductase, methane, activation, marine, fad, [4fe-4s]-cluster, ferredoxin, electron transfer |
Biological source | Methanothermococcus thermolithotrophicus DSM 2095 More |
Total number of polymer chains | 4 |
Total formula weight | 156650.47 |
Authors | Jespersen, M.,Wagner, T. (deposition date: 2022-06-23, release date: 2023-04-26, Last modification date: 2023-11-15) |
Primary citation | Jespersen, M.,Wagner, T. Assimilatory sulfate reduction in the marine methanogen Methanothermococcus thermolithotrophicus. Nat Microbiol, 8:1227-1239, 2023 Cited by PubMed Abstract: Methanothermococcus thermolithotrophicus is the only known methanogen that grows on sulfate as its sole sulfur source, uniquely uniting methanogenesis and sulfate reduction. Here we use physiological, biochemical and structural analyses to provide a snapshot of the complete sulfate reduction pathway of this methanogenic archaeon. We find that later steps in this pathway are catalysed by atypical enzymes. PAPS (3'-phosphoadenosine 5'-phosphosulfate) released by APS kinase is converted into sulfite and 3'-phosphoadenosine 5'-phosphate (PAP) by a PAPS reductase that is similar to the APS reductases of dissimilatory sulfate reduction. A non-canonical PAP phosphatase then hydrolyses PAP. Finally, the F-dependent sulfite reductase converts sulfite to sulfide for cellular assimilation. While metagenomic and metatranscriptomic studies suggest that the sulfate reduction pathway is present in several methanogens, the sulfate assimilation pathway in M. thermolithotrophicus is distinct. We propose that this pathway was 'mix-and-matched' through the acquisition of assimilatory and dissimilatory enzymes from other microorganisms and then repurposed to fill a unique metabolic role. PubMed: 37277534DOI: 10.1038/s41564-023-01398-8 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.45 Å) |
Structure validation
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