8A2C

The crystal structure of the S178A mutant of PET40, a PETase enzyme from an unclassified Amycolatopsis

Summary for 8A2C

• Entry DOI: 10.2210/pdb8a2c/pdb
• Descriptor: PET40 S178A, 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID, 1,2-ETHANEDIOL, ... (6 entities in total)
• Functional Keywords: petase, pet, enzyme, hydrolase, esterase, plastic, degradation, biotech, enzyme evolution
• Biological source: unclassified Amycolatopsis
• Total number of polymer chains: 2
• Total formula weight: 60382.20

Authors

Costanzi, E.,Applegate, V.,Port, A.,Smits, S.H.J. (deposition date: 2022-06-03, release date: 2023-06-14, Last modification date: 2024-06-26)

• Primary citation: Zhang, H.,Dierkes, R.F.,Perez-Garcia, P.,Costanzi, E.,Dittrich, J.,Cea, P.A.,Gurschke, M.,Applegate, V.,Partus, K.,Schmeisser, C.,Pfleger, C.,Gohlke, H.,Smits, S.H.J.,Chow, J.,Streit, W.R.; The metagenome-derived esterase PET40 is highly promiscuous and hydrolyses polyethylene terephthalate (PET).; Febs J., 291:70-91, 2024; PubMed: 37549040; DOI: 10.1111/febs.16924

Experimental method

X-RAY DIFFRACTION (1.6 Å)