8A1S の概要
| エントリーDOI | 10.2210/pdb8a1s/pdb |
| EMDBエントリー | 10134 10135 15072 15086 |
| 分子名称 | Macrophage-expressed gene 1 protein, 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total) |
| 機能のキーワード | pore forming protein, macpf, beta-barrel, immunity, immune system |
| 由来する生物種 | Mus musculus (house mouse) |
| タンパク質・核酸の鎖数 | 16 |
| 化学式量合計 | 1145296.32 |
| 構造登録者 | |
| 主引用文献 | Yu, X.,Ni, T.,Munson, G.,Zhang, P.,Gilbert, R.J.C. Cryo-EM structures of perforin-2 in isolation and assembled on a membrane suggest a mechanism for pore formation. Embo J., 41:e111857-e111857, 2022 Cited by PubMed Abstract: Perforin-2 (PFN2, MPEG1) is a key pore-forming protein in mammalian innate immunity restricting intracellular bacteria proliferation. It forms a membrane-bound pre-pore complex that converts to a pore-forming structure upon acidification; but its mechanism of conformational transition has been debated. Here we used cryo-electron microscopy, tomography and subtomogram averaging to determine structures of PFN2 in pre-pore and pore conformations in isolation and bound to liposomes. In isolation and upon acidification, the pre-assembled complete pre-pore rings convert to pores in both flat ring and twisted conformations. On membranes, in situ assembled PFN2 pre-pores display various degrees of completeness; whereas PFN2 pores are mainly incomplete arc structures that follow the same subunit packing arrangements as found in isolation. Both assemblies on membranes use their P2 β-hairpin for binding to the lipid membrane surface. Overall, these structural snapshots suggest a molecular mechanism for PFN2 pre-pore to pore transition on a targeted membrane, potentially using the twisted pore as an intermediate or alternative state to the flat conformation, with the capacity to cause bilayer distortion during membrane insertion. PubMed: 36245269DOI: 10.15252/embj.2022111857 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (4 Å) |
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