8A18

1.63 A resolution hydroquinone inhibited Sporosarcina pasteurii urease

This is a non-PDB format compatible entry.

Summary for 8A18

• Entry DOI: 10.2210/pdb8a18/pdb
• Descriptor: Urease subunit gamma, Urease subunit beta, Urease subunit alpha, ... (9 entities in total)
• Functional Keywords: urease, nickel, enzyme inhibition, hydroquinone, hydrolase
• Biological source: Sporosarcina pasteurii; More
• Total number of polymer chains: 3
• Total formula weight: 89468.65

Authors

Mazzei, L.,Ciurli, S.,Cianci, M. (deposition date: 2022-06-01, release date: 2023-06-14, Last modification date: 2024-02-07)

Primary citation

Mazzei, L.,Cianci, M.,Ciurli, S.
Inhibition of Urease by Hydroquinones: A Structural and Kinetic Study.
Chemistry, 28:e202201770-e202201770, 2022

PubMed Abstract: Hydroquinones are a class of organic compounds abundant in nature that result from the full reduction of the corresponding quinones. Quinones are known to efficiently inhibit urease, a Ni -containing enzyme that catalyzes the hydrolysis of urea to yield ammonia and carbonate and acts as a virulence factor of several human pathogens, in addition to decreasing the efficiency of soil organic nitrogen fertilization. Here, we report the molecular characterization of the inhibition of urease from Sporosarcina pasteurii (SPU) and Canavalia ensiformis (jack bean, JBU) by 1,4-hydroquinone (HQ) and its methyl and tert-butyl derivatives. The 1.63-Å resolution X-ray crystal structure of the SPU-HQ complex discloses that HQ covalently binds to the thiol group of αCys322, a key residue located on a mobile protein flap directly involved in the catalytic mechanism. Inhibition kinetic data obtained for the three compounds on JBU reveals the occurrence of an irreversible inactivation process that involves a radical-based autocatalytic mechanism.

PubMed: 35994380
DOI: 10.1002/chem.202201770

Experimental method

X-RAY DIFFRACTION (1.63 Å)