8FX6

Non-ribosomal PCP-C didomain (amide stabilised leucine) acceptor bound state

Summary for 8FX6

• Entry DOI: 10.2210/pdb8fx6/pdb
• Descriptor: PCP-C didomain, N-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl]-L-leucinamide (3 entities in total)
• Functional Keywords: nrps, c-domain, pcp-domain, biosynthetic protein
• Biological source: Thermobifida fusca
• Total number of polymer chains: 2
• Total formula weight: 114487.25

Authors

Ho, Y.T.C.,Cryle, M.J. (deposition date: 2023-01-24, release date: 2023-06-21, Last modification date: 2024-11-13)

Primary citation

Ho, Y.T.C.,Kaczmarski, J.A.,Tailhades, J.,Izore, T.,Steer, D.L.,Schittenhelm, R.B.,Tosin, M.,Jackson, C.J.,Cryle, M.J.
Not always an innocent bystander: the impact of stabilised phosphopantetheine moieties when studying nonribosomal peptide biosynthesis.
Chem.Commun.(Camb.), 59:8234-8237, 2023

PubMed Abstract: Nonribosomal peptide synthetases produce many important peptide natural products and are centred around carrier proteins (CPs) that deliver intermediates to various catalytic domains. We show that the replacement of CP substrate thioesters by stabilised ester analogues leads to active condensation domain complexes, whereas amide stabilisation generates non-functional complexes.

PubMed: 37310188
DOI: 10.1039/d3cc01578e

Experimental method

X-RAY DIFFRACTION (2.2 Å)