7ZXE

Structure of SNAPc containing Pol II pre-initiation complex bound to U1 snRNA promoter (OC)

7ZXE の概要

• エントリーDOI: 10.2210/pdb7zxe/pdb
• EMDBエントリー: 15009
• 分子名称: Transcription initiation factor IIB, snRNA-activating protein complex subunit 5, ZINC ION, ... (11 entities in total)
• 機能のキーワード: rna polymerase ii, pol ii, pic, snapc, snrna, u1 promoter, transcription
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 446858.88

構造登録者

Rengachari, S.,Schilbach, S.,Kaliyappan, T.,Gouge, J.,Zumer, K.,Schwarz, J.,Urlaub, H.,Dienemann, C.,Vannini, A.,Cramer, P. (登録日: 2022-05-20, 公開日: 2022-11-30, 最終更新日: 2024-07-24)

主引用文献

Rengachari, S.,Schilbach, S.,Kaliyappan, T.,Gouge, J.,Zumer, K.,Schwarz, J.,Urlaub, H.,Dienemann, C.,Vannini, A.,Cramer, P.
Structural basis of SNAPc-dependent snRNA transcription initiation by RNA polymerase II.
Nat.Struct.Mol.Biol., 29:1159-1169, 2022

PubMed Abstract: RNA polymerase II (Pol II) carries out transcription of both protein-coding and non-coding genes. Whereas Pol II initiation at protein-coding genes has been studied in detail, Pol II initiation at non-coding genes, such as small nuclear RNA (snRNA) genes, is less well understood at the structural level. Here, we study Pol II initiation at snRNA gene promoters and show that the snRNA-activating protein complex (SNAPc) enables DNA opening and transcription initiation independent of TFIIE and TFIIH in vitro. We then resolve cryo-EM structures of the SNAPc-containing Pol IIpre-initiation complex (PIC) assembled on U1 and U5 snRNA promoters. The core of SNAPc binds two turns of DNA and recognizes the snRNA promoter-specific proximal sequence element (PSE), located upstream of the TATA box-binding protein TBP. Two extensions of SNAPc, called wing-1 and wing-2, bind TFIIA and TFIIB, respectively, explaining how SNAPc directs Pol II to snRNA promoters. Comparison of structures of closed and open promoter complexes elucidates TFIIH-independent DNA opening. These results provide the structural basis of Pol II initiation at non-coding RNA gene promoters.

PubMed: 36424526
DOI: 10.1038/s41594-022-00857-w

実験手法

ELECTRON MICROSCOPY (3.5 Å)