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7ZW1

Human PRPH2-ROM1 hetero-dimer

Summary for 7ZW1
Entry DOI10.2210/pdb7zw1/pdb
EMDB information14991
DescriptorPeripherin-2, Rod outer segment membrane protein 1, Nanobody (3 entities in total)
Functional Keywordsprph2, peripherin-2, rom1, hetero-complex, membrane protein, tetraspanin
Biological sourceHomo sapiens (human)
More
Total number of polymer chains3
Total formula weight91950.10
Authors
El Mazouni, D.,Gros, P. (deposition date: 2022-05-17, release date: 2022-11-16)
Primary citationEl Mazouni, D.,Gros, P.
Cryo-EM structures of peripherin-2 and ROM1 suggest multiple roles in photoreceptor membrane morphogenesis.
Sci Adv, 8:eadd3677-eadd3677, 2022
Cited by
PubMed Abstract: Mammalian peripherin-2 (PRPH2) and rod outer segment membrane protein 1 (ROM1) are retina-specific tetraspanins that partake in the constant renewal of stacked membrane discs of photoreceptor cells that enable vision. Here, we present single-particle cryo-electron microscopy structures of solubilized PRPH2-ROM1 heterodimers and higher-order oligomers. High-risk PRPH2 and ROM1 mutations causing blindness map to the protein-dimer interface. Cysteine bridges connect dimers forming positive-curved oligomers, whereas negative-curved oligomers were observed occasionally. Hexamers and octamers exhibit a secondary micelle that envelopes four carboxyl-terminal helices, supporting a potential role in membrane remodeling. Together, the data indicate multiple structures for PRPH2-ROM1 in creating and maintaining compartmentalization of photoreceptor cells.
PubMed: 36351012
DOI: 10.1126/sciadv.add3677
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (3.7 Å)
Structure validation

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