7ZVY

Thermococcus kadokarensis phosphomannose isomerase

Summary for 7ZVY

• Entry DOI: 10.2210/pdb7zvy/pdb
• Descriptor: Cupin_2 domain-containing protein, ZINC ION, ... (6 entities in total)
• Functional Keywords: high pressure adaptation, proteins dynamics, neutron scattering, isomerase
• Biological source: Thermococcus kodakarensis; More
• Total number of polymer chains: 8
• Total formula weight: 105907.88

Authors

Hoh, f.,Calio, A. (deposition date: 2022-05-17, release date: 2022-08-24, Last modification date: 2024-05-01)

Primary citation

Calio, A.,Dubois, C.,Fontanay, S.,Koza, M.M.,Hoh, F.,Roumestand, C.,Oger, P.,Peters, J.
Unravelling the Adaptation Mechanisms to High Pressure in Proteins.
Int J Mol Sci, 23:-, 2022

PubMed Abstract: Life is thought to have appeared in the depth of the sea under high hydrostatic pressure. Nowadays, it is known that the deep biosphere hosts a myriad of life forms thriving under high-pressure conditions. However, the evolutionary mechanisms leading to their adaptation are still not known. Here, we show the molecular bases of these mechanisms through a joint structural and dynamical study of two orthologous proteins. We observed that pressure adaptation involves the decoupling of protein-water dynamics and the elimination of cavities in the protein core. This is achieved by rearranging the charged residues on the protein surface and using bulkier hydrophobic residues in the core. These findings will be the starting point in the search for a complete genomic model explaining high-pressure adaptation.

PubMed: 35955607
DOI: 10.3390/ijms23158469

Experimental method

X-RAY DIFFRACTION (2.16 Å)