7ZKP

Late assembly intermediate of the proximal proton pumping module of complex I with assembly factors NDUFAF1 and CIA84

7ZKP の概要

• エントリーDOI: 10.2210/pdb7zkp/pdb
• EMDBエントリー: 14764
• 分子名称: Subunit NIMM of NADH:Ubiquinone Oxidoreductase (Complex I), subunit NI9M of protein NADH:Ubiquinone Oxidoreductase (Complex I), Subunit NEBM of NADH:Ubiquinone Oxidoreductase (Complex I), ... (19 entities in total)
• 機能のキーワード: assembly respiratory chain membrane protein mitochondria, electron transport
• 由来する生物種: Yarrowia lipolytica; 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 361535.91

構造登録者

Schiller, J.,Laube, E.,Vonck, J.,Zickermann, V. (登録日: 2022-04-13, 公開日: 2022-11-23, 最終更新日: 2024-11-13)

主引用文献

Schiller, J.,Laube, E.,Wittig, I.,Kuhlbrandt, W.,Vonck, J.,Zickermann, V.
Insights into complex I assembly: Function of NDUFAF1 and a link with cardiolipin remodeling.
Sci Adv, 8:eadd3855-eadd3855, 2022

PubMed Abstract: Respiratory complex I is a ~1-MDa proton pump in mitochondria. Its structure has been revealed in great detail, but the structural basis of its assembly, in humans involving at least 15 assembly factors, is essentially unknown. We determined cryo-electron microscopy structures of assembly intermediates associated with assembly factor NDUFAF1 in a yeast model system. Subunits ND2 and NDUFC2 together with assembly factors NDUFAF1 and CIA84 form the nucleation point of the NDUFAF1-dependent assembly pathway. Unexpectedly, the cardiolipin remodeling enzyme tafazzin is an integral component of this core complex. In a later intermediate, all 12 subunits of the proximal proton pump module have assembled. NDUFAF1 locks the central ND3 subunit in an assembly-competent conformation, and major rearrangements of central subunits are required for complex I maturation.

PubMed: 36383672
DOI: 10.1126/sciadv.add3855

実験手法

ELECTRON MICROSCOPY (3.2 Å)