7ZJS

Structural basis of centromeric cohesion protection by SGO1

7ZJS の概要

• エントリーDOI: 10.2210/pdb7zjs/pdb
• 分子名称: Cohesin subunit SA-2, Double-strand-break repair protein rad21 homolog, Shugoshin 1, ... (4 entities in total)
• 機能のキーワード: cohesin, stag2, sgo1, heat, cell cycle
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 429179.49

構造登録者

Patel, A.,Panne, D. (登録日: 2022-04-11, 公開日: 2023-04-26, 最終更新日: 2024-02-07)

主引用文献

Garcia-Nieto, A.,Patel, A.,Li, Y.,Oldenkamp, R.,Feletto, L.,Graham, J.J.,Willems, L.,Muir, K.W.,Panne, D.,Rowland, B.D.
Structural basis of centromeric cohesion protection.
Nat.Struct.Mol.Biol., 30:853-859, 2023

PubMed Abstract: In the early stages of mitosis, cohesin is released from chromosome arms but not from centromeres. The protection of centromeric cohesin by SGO1 maintains the sister chromatid cohesion that resists the pulling forces of microtubules until all chromosomes are attached in a bipolar manner to the mitotic spindle. Here we present the X-ray crystal structure of a segment of human SGO1 bound to a conserved surface of the cohesin complex. SGO1 binds to a composite interface formed by the SA2 and SCC1 subunits of cohesin. SGO1 shares this binding interface with CTCF, indicating that these distinct chromosomal regulators control cohesin through a universal principle. This interaction is essential for the localization of SGO1 to centromeres and protects centromeric cohesin against WAPL-mediated cohesin release. SGO1-cohesin binding is maintained until the formation of microtubule-kinetochore attachments and is required for faithful chromosome segregation and the maintenance of a stable karyotype.

PubMed: 37081319
DOI: 10.1038/s41594-023-00968-y

実験手法

X-RAY DIFFRACTION (3.24 Å)