7ZGR

Polymerase module of yeast CPF in complex with Mpe1, the yPIM of Cft2 and the pre-cleaved CYC1 RNA

7ZGR の概要

• エントリーDOI: 10.2210/pdb7zgr/pdb
• 関連するPDBエントリー: 7ZGP 7ZGQ
• EMDBエントリー: 14710 14711 14712
• 分子名称: Protein CFT1, mRNA 3'-end-processing protein YTH1, Cleavage factor two protein 2, ... (7 entities in total)
• 機能のキーワード: cpf, 3'-end processing, rna binding protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 375548.29

構造登録者

Rodriguez-Molina, J.B.,Passmore, L.A. (登録日: 2022-04-04, 公開日: 2022-05-25, 最終更新日: 2024-07-24)

主引用文献

Rodriguez-Molina, J.B.,O'Reilly, F.J.,Fagarasan, H.,Sheekey, E.,Maslen, S.,Skehel, J.M.,Rappsilber, J.,Passmore, L.A.
Mpe1 senses the binding of pre-mRNA and controls 3' end processing by CPF.
Mol.Cell, 82:2490-, 2022

PubMed Abstract: Most eukaryotic messenger RNAs (mRNAs) are processed at their 3' end by the cleavage and polyadenylation specificity factor (CPF/CPSF). CPF mediates the endonucleolytic cleavage of the pre-mRNA and addition of a polyadenosine (poly(A)) tail, which together define the 3' end of the mature transcript. The activation of CPF is highly regulated to maintain the fidelity of RNA processing. Here, using cryo-EM of yeast CPF, we show that the Mpe1 subunit directly contacts the polyadenylation signal sequence in nascent pre-mRNA. The region of Mpe1 that contacts RNA also promotes the activation of CPF endonuclease activity and controls polyadenylation. The Cft2 subunit of CPF antagonizes the RNA-stabilized configuration of Mpe1. In vivo, the depletion or mutation of Mpe1 leads to widespread defects in transcription termination by RNA polymerase II, resulting in transcription interference on neighboring genes. Together, our data suggest that Mpe1 plays a major role in accurate 3' end processing, activating CPF, and ensuring timely transcription termination.

PubMed: 35584695
DOI: 10.1016/j.molcel.2022.04.021

実験手法

ELECTRON MICROSCOPY (2.6 Å)