7Z6Q

Cryo-EM structure of the whole photosynthetic complex from the green sulfur bacteria

Summary for 7Z6Q

• Entry DOI: 10.2210/pdb7z6q/pdb
• EMDB information: 14528
• Descriptor: Photosystem P840 reaction center, large subunit, 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE, [(2~{R})-2-hexadecanoyloxy-3-[(2~{S},3~{S},4~{R},5~{R},6~{S})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-propyl] hexadecanoate, ... (14 entities in total)
• Functional Keywords: reaction centre, electron transport, energy transfer, green sulfur bacterium, membrane protein, light-harvesting protein complex, photosynthesis
• Biological source: Chlorobaculum tepidum TLS; More
• Total number of polymer chains: 12
• Total formula weight: 569591.81

Authors

Xie, H.,Tsiotis, G. (deposition date: 2022-03-14, release date: 2023-02-08, Last modification date: 2025-10-01)

Primary citation

Xie, H.,Lyratzakis, A.,Khera, R.,Koutantou, M.,Welsch, S.,Michel, H.,Tsiotis, G.
Cryo-EM structure of the whole photosynthetic reaction center apparatus from the green sulfur bacterium Chlorobaculum tepidum.
Proc.Natl.Acad.Sci.USA, 120:e2216734120-e2216734120, 2023

PubMed Abstract: Light energy absorption and transfer are very important processes in photosynthesis. In green sulfur bacteria light is absorbed primarily by the chlorosomes and its energy is transferred via the Fenna-Matthews-Olson (FMO) proteins to a homodimeric reaction center (RC). Here, we report the cryogenic electron microscopic structure of the intact FMO-RC apparatus from at 2.5 Å resolution. The FMO-RC apparatus presents an asymmetric architecture and contains two FMO trimers that show different interaction patterns with the RC core. Furthermore, the two permanently bound transmembrane subunits PscC, which donate electrons to the special pair, interact only with the two large PscA subunits. This structure fills an important gap in our understanding of the transfer of energy from antenna to the electron transport chain of this RC and the transfer of electrons from reduced sulfur compounds to the special pair.

PubMed: 36693097
DOI: 10.1073/pnas.2216734120

Experimental method

ELECTRON MICROSCOPY (2.5 Å)