7Z4E

SpCas9 bound to 8-nucleotide complementary DNA substrate

Summary for 7Z4E

Entry DOI	10.2210/pdb7z4e/pdb
EMDB information	14494
Descriptor	CRISPR-associated endonuclease Cas9/Csn1, sgRNA, Target strand of 8 nucleotide complementary DNA substrate, ... (4 entities in total)
Functional Keywords	crispr, cas9, r-loop, substrate binding, off-target, hydrolase
Biological source	Streptococcus pyogenes More
Total number of polymer chains	4
Total formula weight	209989.31
Authors	Pacesa, M.,Jinek, M. (deposition date: 2022-03-03, release date: 2022-08-31, Last modification date: 2022-09-14)
Primary citation	Pacesa, M.,Loeff, L.,Querques, I.,Muckenfuss, L.M.,Sawicka, M.,Jinek, M. R-loop formation and conformational activation mechanisms of Cas9. Nature, 609:191-196, 2022 Cited by PubMed: 36002571 DOI: 10.1038/s41586-022-05114-0 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (4.14 Å)

Structure validation