7Z02

Z-SBTub2M photoswitch bound to tubulin-DARPin D1 complex

Summary for 7Z02

Entry DOI	10.2210/pdb7z02/pdb
Descriptor	Tubulin alpha-1B chain, Tubulin beta-2B chain, Designed Ankyrin Repeat Protein (DARPIN) D1, ... (8 entities in total)
Functional Keywords	microtubule dynamics; cytoskeleton; photopharmacology; tubulin polymerisation inhibitor; optical control; colchicine; antimitotic; cell division; cell migration; development; photoswitch, cell cycle
Biological source	synthetic construct More
Total number of polymer chains	3
Total formula weight	119631.20
Authors	Wranik, M.,Weinert, T.,Standfuss, J.,Steinmetz, M. (deposition date: 2022-02-21, release date: 2022-03-30, Last modification date: 2024-01-31)
Primary citation	Gao, L.,Meiring, J.C.M.,Varady, A.,Ruider, I.E.,Heise, C.,Wranik, M.,Velasco, C.D.,Taylor, J.A.,Terni, B.,Weinert, T.,Standfuss, J.,Cabernard, C.C.,Llobet, A.,Steinmetz, M.O.,Bausch, A.R.,Distel, M.,Thorn-Seshold, J.,Akhmanova, A.,Thorn-Seshold, O. In Vivo Photocontrol of Microtubule Dynamics and Integrity, Migration and Mitosis, by the Potent GFP-Imaging-Compatible Photoswitchable Reagents SBTubA4P and SBTub2M. J.Am.Chem.Soc., 144:5614-5628, 2022 Cited by PubMed: 35290733 DOI: 10.1021/jacs.2c01020 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.36 Å)

Structure validation