7YYO
Cryo-EM structure of an a-carboxysome RuBisCO enzyme at 2.9 A resolution
Summary for 7YYO
| Entry DOI | 10.2210/pdb7yyo/pdb |
| EMDB information | 14385 |
| Descriptor | Ribulose bisphosphate carboxylase large chain, Ribulose bisphosphate carboxylase small chain, MAGNESIUM ION, ... (4 entities in total) |
| Functional Keywords | carboxysome, carbon fixation, cyanobacteria, photosynthesis |
| Biological source | Cyanobium More |
| Total number of polymer chains | 16 |
| Total formula weight | 526996.50 |
| Authors | Mann, D.,Evans, S.L.,Bergeron, J.R.C. (deposition date: 2022-02-18, release date: 2023-01-25, Last modification date: 2025-10-01) |
| Primary citation | Evans, S.L.,Al-Hazeem, M.M.J.,Mann, D.,Smetacek, N.,Beavil, A.J.,Sun, Y.,Chen, T.,Dykes, G.F.,Liu, L.N.,Bergeron, J.R.C. Single-particle cryo-EM analysis of the shell architecture and internal organization of an intact alpha-carboxysome. Structure, 31:677-, 2023 Cited by PubMed Abstract: Carboxysomes are proteinaceous bacterial microcompartments that sequester the key enzymes for carbon fixation in cyanobacteria and some proteobacteria. They consist of a virus-like icosahedral shell, encapsulating several enzymes, including ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), responsible for the first step of the Calvin-Benson-Bassham cycle. Despite their significance in carbon fixation and great bioengineering potentials, the structural understanding of native carboxysomes is currently limited to low-resolution studies. Here, we report the characterization of a native α-carboxysome from a marine cyanobacterium by single-particle cryoelectron microscopy (cryo-EM). We have determined the structure of its RuBisCO enzyme, and obtained low-resolution maps of its icosahedral shell, and of its concentric interior organization. Using integrative modeling approaches, we have proposed a complete atomic model of an intact carboxysome, providing insight into its organization and assembly. This is critical for a better understanding of the carbon fixation mechanism and toward repurposing carboxysomes in synthetic biology for biotechnological applications. PubMed: 37015227DOI: 10.1016/j.str.2023.03.008 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.87 Å) |
Structure validation
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