7YUL

Crystal structure of human BEND6 BEN domain in complex with DNA

Summary for 7YUL

• Entry DOI: 10.2210/pdb7yul/pdb
• Descriptor: BEN domain-containing protein 6, DNA (5'-D(*CP*TP*CP*TP*CP*GP*CP*GP*AP*GP*AP*G)-3'), GLYCOLIC ACID, ... (4 entities in total)
• Functional Keywords: complex structure, dna binding protein
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 15545.99

Authors

Liu, K.,Xiao, Y.Q.,Zhang, J.,Min, J.R. (deposition date: 2022-08-17, release date: 2023-04-26, Last modification date: 2023-11-29)

Primary citation

Liu, K.,Zhang, J.,Xiao, Y.,Yang, A.,Song, X.,Li, Y.,Chen, Y.,Hughes, T.R.,Min, J.
Structural insights into DNA recognition by the BEN domain of the transcription factor BANP.
J.Biol.Chem., 299:104734-104734, 2023

PubMed Abstract: The BEN domain-containing transcription factors regulate transcription by recruiting chromatin-modifying factors to specific chromatin regions via their DNA-binding BEN domains. The BEN domain of BANP has been shown to bind to a CGCG DNA sequence or an AAA-containing matrix attachment regions DNA sequence. Consistent with these in vivo observations, we identified an optimal DNA-binding sequence of AAATCTCG by protein binding microarray, which was also confirmed by our isothermal titration calorimetry and mutagenesis results. We then determined crystal structures of the BANP BEN domain in apo form and in complex with a CGCG-containing DNA, respectively, which revealed that the BANP BEN domain mainly used the electrostatic interactions to bind DNA with some base-specific interactions with the TC motifs. Our isothermal titration calorimetry results also showed that BANP bound to unmethylated and methylated DNAs with comparable binding affinities. Our complex structure of BANP-mCGCG revealed that the BANP BEN domain bound to the unmethylated and methylated DNAs in a similar mode and cytosine methylation did not get involved in binding, which is also consistent with our observations from the complex structures of the BEND6 BEN domain with the CGCG or CGmCG DNAs. Taken together, our results further elucidate the elements important for DNA recognition and transcriptional regulation by the BANP BEN domain-containing transcription factor.

PubMed: 37086783
DOI: 10.1016/j.jbc.2023.104734

Experimental method

X-RAY DIFFRACTION (1.82 Å)