7YO8

Crystal structure of fission yeast Hfl1 LIR fused to human GABARAPL2

Summary for 7YO8

• Entry DOI: 10.2210/pdb7yo8/pdb
• Descriptor: Transmembrane protein 184 homolog C30D11.06c,Gamma-aminobutyric acid receptor-associated protein-like 2 (2 entities in total)
• Functional Keywords: autophagy, vacuole, autophagosome, protein transport
• Biological source: Schizosaccharomyces pombe (fission yeast); More
• Total number of polymer chains: 1
• Total formula weight: 17252.77

Authors

Yamasaki, A.,Noda, N.N. (deposition date: 2022-08-01, release date: 2022-11-02, Last modification date: 2023-11-29)

Primary citation

Park, S.W.,Jeon, P.,Yamasaki, A.,Lee, H.E.,Choi, H.,Mun, J.Y.,Jun, Y.W.,Park, J.H.,Lee, S.H.,Lee, S.K.,Lee, Y.K.,Song, H.K.,Lazarou, M.,Cho, D.H.,Komatsu, M.,Noda, N.N.,Jang, D.J.,Lee, J.A.
Development of new tools to study membrane-anchored mammalian Atg8 proteins.
Autophagy, 19:1424-1443, 2023

PubMed Abstract: A:C autophagic membrane:cytosol; ALS amyotrophic lateral sclerosis; ATG4 autophagy related 4; Atg8 autophagy related 8; BafA1 bafilomycin A; BNIP3L/Nix BCL2 interacting protein 3 like; CALCOCO2/NDP52 calcium binding and coiled-coil domain 2; EBSS Earle's balanced salt solution; GABARAP GABA type A receptor-associated protein; GST glutathione S transferase; HKO hexa knockout; K dissociation constant; LIR LC3-interacting region; MAP1LC3/LC3 microtubule associated protein 1 light chain 3; NLS nuclear localization signal/sequence; PE phosphatidylethanolamine; SpHfl1 organic solute transmembrane transporter; SQSTM1/p62 SQSTM1/p62; TARDBP/TDP-43 TAR DNA binding protein; TKO triple knockout.

PubMed: 36250672
DOI: 10.1080/15548627.2022.2132040

Experimental method

X-RAY DIFFRACTION (1.805 Å)