Summary for 7YFZ
| Entry DOI | 10.2210/pdb7yfz/pdb |
| EMDB information | 33802 |
| Descriptor | Pam3 baseplate wedge gp22, Pam3 baseplate wedge gp23, Pam3 sheath initiator gp21, ... (7 entities in total) |
| Functional Keywords | baseplate, virus, viral protein |
| Biological source | uncultured cyanophage More |
| Total number of polymer chains | 42 |
| Total formula weight | 968437.91 |
| Authors | Yang, F.,Jiang, Y.L.,Zhou, C.Z. (deposition date: 2022-07-09, release date: 2023-01-18, Last modification date: 2024-10-30) |
| Primary citation | Yang, F.,Jiang, Y.L.,Zhang, J.T.,Zhu, J.,Du, K.,Yu, R.C.,Wei, Z.L.,Kong, W.W.,Cui, N.,Li, W.F.,Chen, Y.,Li, Q.,Zhou, C.Z. Fine structure and assembly pattern of a minimal myophage Pam3. Proc.Natl.Acad.Sci.USA, 120:e2213727120-e2213727120, 2023 Cited by PubMed Abstract: The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail contraction mechanism of myophage remain largely unknown. Here, we present the fine structure of a freshwater cyanophage Pam3, which has an icosahedral capsid of ~680 Å in diameter, connected via a three-section neck to an 840-Å-long contractile tail, ending with a three-module baseplate composed of only six protein components. This simplified baseplate consists of a central hub-spike surrounded by six wedge heterotriplexes, to which twelve tail fibers are covalently attached via disulfide bonds in alternating upward and downward configurations. In vitro reduction assays revealed a putative redox-dependent mechanism of baseplate assembly and tail sheath contraction. These findings establish a minimal myophage that might become a user-friendly chassis phage in synthetic biology. PubMed: 36656854DOI: 10.1073/pnas.2213727120 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.19 Å) |
Structure validation
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