7YEU
Superfolder green fluorescent protein with phosphine unnatural amino acid P3BF
Summary for 7YEU
| Entry DOI | 10.2210/pdb7yeu/pdb |
| Descriptor | Superfolder green fluorescent protein (2 entities in total) |
| Functional Keywords | unnatural amino acid, fluorescent protein, phosphine, genetic code expansion |
| Biological source | Aequorea victoria |
| Total number of polymer chains | 1 |
| Total formula weight | 27368.55 |
| Authors | Hu, C.,Duan, H.Z.,Liu, X.H.,Chen, Y.X.,Wang, J.Y. (deposition date: 2022-07-06, release date: 2023-07-26, Last modification date: 2026-03-04) |
| Primary citation | Duan, H.Z.,Hu, C.,Li, Y.L.,Wang, S.H.,Xia, Y.,Liu, X.,Wang, J.,Chen, Y.X. Genetically Encoded Phosphine Ligand for Metalloprotein Design. J.Am.Chem.Soc., 144:22831-22837, 2022 Cited by PubMed Abstract: Phosphine ligands are the most important class of ligands for cross-coupling reactions due to their unique electronic and steric properties. However, metalloproteins generally rely on nitrogen, sulfur, or oxygen ligands. Here, we report the genetic incorporation of P3BF, which contains a biocompatible borane-protected phosphine, into proteins. This step is followed by a straightforward one-pot strategy to perform deboronation and palladium coordination in aqueous and aerobic conditions. The genetically encoded phosphine ligand P3BF should significantly expand our ability to design functional metalloproteins. PubMed: 36417425DOI: 10.1021/jacs.2c09683 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.95 Å) |
Structure validation
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