7YEQ
Structural insight into African Swine Fever Virus CP312R protein reveals it as a single-stranded DNA binding protein
Summary for 7YEQ
| Entry DOI | 10.2210/pdb7yeq/pdb |
| Descriptor | CP312R (2 entities in total) |
| Functional Keywords | african swine fever virus, cp312r, single-stranded dna binding protein, ob-fold, virus |
| Biological source | African swine fever virus (ASFV) |
| Total number of polymer chains | 1 |
| Total formula weight | 34532.69 |
| Authors | Chen, L.F.,Wu, Y.K. (deposition date: 2022-07-06, release date: 2023-07-26, Last modification date: 2026-03-04) |
| Primary citation | Chen, L.,Chen, L.,Chen, H.,Zhang, H.,Dong, P.,Sun, L.,Huang, X.,Lin, P.,Wu, L.,Jing, D.,Qian, Y.,Wu, Y. Structural insights into the CP312R protein of the African swine fever virus. Biochem.Biophys.Res.Commun., 624:68-74, 2022 Cited by PubMed Abstract: African swine fever (ASF) is a lethal hemorrhagic disease that affects domestic pigs and wild boars. There is no medication available for ASF to date. The ability to mount antigen-specific responses to viral vectored CP312R makes it a crucial potential target for designing vaccines or drugs. This study determined the crystal structure of ASFV CP312R at 2.32 Å and found it to be a monomer with a single-stranded DNA binding core domain with a clear five-strands β-barrel OB-fold architecture. Electrophoretic mobility shift assay and size-exclusion chromatography characterization assay further confirmed the single-stranded DNA (ssDNA)-binding property of ASFV CP312R. This study revealed the structure and preliminary ssDNA interaction mechanisms of ASFV CP312R, providing new clues for developing new antiviral strategies. PubMed: 35933928DOI: 10.1016/j.bbrc.2022.07.091 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.32 Å) |
Structure validation
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