7Y86

CcpS mutant

Summary for 7Y86

• Entry DOI: 10.2210/pdb7y86/pdb
• Descriptor: UPF0297 protein A7J08_00425 (2 entities in total)
• Functional Keywords: ccps, mutant, gene regulation
• Biological source: Streptococcus suis
• Total number of polymer chains: 2
• Total formula weight: 20999.34

Authors

Tang, J.S.,Ran, T.T.,Wang, W.W.,Fan, H.J. (deposition date: 2022-06-22, release date: 2023-05-10, Last modification date: 2023-11-29)

Primary citation

Tang, J.,Guo, M.,Chen, M.,Xu, B.,Ran, T.,Wang, W.,Ma, Z.,Lin, H.,Fan, H.
A link between STK signalling and capsular polysaccharide synthesis in Streptococcus suis.
Nat Commun, 14:2480-2480, 2023

PubMed Abstract: Synthesis of capsular polysaccharide (CPS), an important virulence factor of pathogenic bacteria, is modulated by the CpsBCD phosphoregulatory system in Streptococcus. Serine/threonine kinases (STKs, e.g. Stk1) can also regulate CPS synthesis, but the underlying mechanisms are unclear. Here, we identify a protein (CcpS) that is phosphorylated by Stk1 and modulates the activity of phosphatase CpsB in Streptococcus suis, thus linking Stk1 to CPS synthesis. The crystal structure of CcpS shows an intrinsically disordered region at its N-terminus, including two threonine residues that are phosphorylated by Stk1. The activity of phosphatase CpsB is inhibited when bound to non-phosphorylated CcpS. Thus, CcpS modulates the activity of phosphatase CpsB thereby altering CpsD phosphorylation, which in turn modulates the expression of the Wzx-Wzy pathway and thus CPS production.

PubMed: 37120581
DOI: 10.1038/s41467-023-38210-4

Experimental method

X-RAY DIFFRACTION (1.5 Å)