7Y77
Crystal structure of rice NAL1
Summary for 7Y77
| Entry DOI | 10.2210/pdb7y77/pdb |
| Descriptor | Protein NARROW LEAF 1, ADENOSINE-5'-TRIPHOSPHATE (3 entities in total) |
| Functional Keywords | serine protease, nal1, hydrolase |
| Biological source | Oryza sativa (Asian cultivated rice) |
| Total number of polymer chains | 3 |
| Total formula weight | 144058.74 |
| Authors | Yan, J.J.,Guan, Z.Y.,Yin, P.,Xiong, L.Z. (deposition date: 2022-06-21, release date: 2023-07-12, Last modification date: 2024-05-29) |
| Primary citation | Li, W.,Yan, J.,Zhang, Y.,Zhang, F.,Guan, Z.,Yao, Y.,Chang, Y.,Tu, H.,Li, X.,Wang, H.,Xiong, H.,Lai, X.,Yin, P.,Xiong, L. Serine protease NAL1 exerts pleiotropic functions through degradation of TOPLESS-related corepressor in rice. Nat.Plants, 9:1130-1142, 2023 Cited by PubMed Abstract: NARROW LEAF 1 (NAL1) is a breeding-valuable pleiotropic gene that affects multiple agronomic traits in rice, although the molecular mechanism is largely unclear. Here, we report that NAL1 is a serine protease and displays a novel hexameric structure consisting of two ATP-mediated doughnut-shaped trimeric complexes. Moreover, we identified TOPLESS-related corepressor OsTPR2 involved in multiple growth and development processes as the substrate of NAL1. We found that NAL1 degraded OsTPR2, thus modulating the expression of downstream genes related to hormone signalling pathways, eventually achieving its pleiotropic physiological function. An elite allele, NAL1, which may have originated from wild rice, could increase grain yield. Furthermore, the NAL1 homologues in different crops have a similar pleiotropic function to NAL1. Our study uncovers a NAL1-OsTPR2 regulatory module and provides gene resources for the design of high-yield crops. PubMed: 37349549DOI: 10.1038/s41477-023-01449-2 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.6 Å) |
Structure validation
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