7Y5U
Cryo-EM structure of the monomeric human CAF1LC-H3-H4 complex
Summary for 7Y5U
Entry DOI | 10.2210/pdb7y5u/pdb |
EMDB information | 33625 |
Descriptor | Chromatin assembly factor 1 subunit A, Histone H3.1, Histone H4, ... (5 entities in total) |
Functional Keywords | histone chaperone, chromatin assembly factor, replication |
Biological source | Homo sapiens (human) More |
Total number of polymer chains | 5 |
Total formula weight | 168484.91 |
Authors | |
Primary citation | Liu, C.P.,Yu, Z.,Xiong, J.,Hu, J.,Song, A.,Ding, D.,Yu, C.,Yang, N.,Wang, M.,Yu, J.,Hou, P.,Zeng, K.,Li, Z.,Zhang, Z.,Zhang, X.,Li, W.,Zhang, Z.,Zhu, B.,Li, G.,Xu, R.M. Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1. Science, 381:eadd8673-eadd8673, 2023 Cited by PubMed Abstract: Chromatin inheritance entails de novo nucleosome assembly after DNA replication by chromatin assembly factor-1 (CAF-1). Yet direct knowledge about CAF-1's histone binding mode and nucleosome assembly process is lacking. In this work, we report the crystal structure of human CAF-1 in the absence of histones and the cryo-electron microscopy structure of CAF-1 in complex with histones H3 and H4. One histone H3-H4 heterodimer is bound by one CAF-1 complex mainly through the p60 subunit and the acidic domain of the p150 subunit. We also observed a dimeric CAF-1-H3-H4 supercomplex in which two H3-H4 heterodimers are poised for tetramer assembly and discovered that CAF-1 facilitates right-handed DNA wrapping of H3-H4 tetramers. These findings signify the involvement of DNA in H3-H4 tetramer formation and suggest a right-handed nucleosome precursor in chromatin replication. PubMed: 37616371DOI: 10.1126/science.add8673 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (3.8 Å) |
Structure validation
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