7XV7

Crystal structure of ZYG11B bound to ORF10 peptide

Summary for 7XV7

• Entry DOI: 10.2210/pdb7xv7/pdb
• Descriptor: Protein zyg-11 homolog B, SULFATE ION (3 entities in total)
• Functional Keywords: e3 ligase, ligase
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 57371.40

Authors

Dong, C.,Yan, X.,Li, Y. (deposition date: 2022-05-21, release date: 2022-06-08, Last modification date: 2024-05-08)

Primary citation

Zhang, B.,Li, Y.,Feng, Q.,Song, L.,Dong, C.,Yan, X.
Structural insights into ORF10 recognition by ZYG11B.
Biochem.Biophys.Res.Commun., 616:14-18, 2022

PubMed Abstract: Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has become a major threat to human health. As a unique putative protein of SARS-CoV-2, the N-terminus of ORF10 can be recognized by ZYG11B, a substrate receptor of the Cullin 2-RING E3 ubiquitin ligase (CRL2). Here we elucidated recognition mechanism of ORF10 N-terminus by ZYG11B through presenting the crystal structure of ZYG11B bound to ORF10 N-terminal peptide. Our work expands the current understanding of ORF10 interaction with ZYG11B, and may also inspire the development of novel therapies for COVID-19.

PubMed: 35636250
DOI: 10.1016/j.bbrc.2022.05.069

Experimental method

X-RAY DIFFRACTION (2.6 Å)