7XRB

human STK19 dimer

7XRB の概要

• エントリーDOI: 10.2210/pdb7xrb/pdb
• 分子名称: Isoform 2 of Serine/threonine-protein kinase 19, SULFATE ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: winged helix domain, nucleus protein, dna binding protein
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 52481.74

構造登録者

Sun, Q.,Li, Y. (登録日: 2022-05-10, 公開日: 2023-06-07, 最終更新日: 2025-02-26)

主引用文献

Li, Y.,Gong, Y.,Zhou, Y.,Xiao, Y.,Huang, W.,Zhou, Q.,Tu, Y.,Zhao, Y.,Zhang, S.,Dai, L.,Sun, Q.
STK19 is a DNA/RNA-binding protein critical for DNA damage repair and cell proliferation.
J.Cell Biol., 223:-, 2024

PubMed Abstract: STK19 was originally identified as a manganese-dependent serine/threonine-specific protein kinase, but its function has been highly debated. Here, the crystal structure of STK19 revealed that it does not contain a kinase domain, but three intimately packed winged helix (WH) domains. The third WH domain mediated homodimerization and double-stranded DNA binding, both being important for its nuclear localization. STK19 participated in the nucleotide excision repair (NER) and mismatch repair (MMR) pathways by recruiting damage repair factors such as RPA2 and PCNA. STK19 also bound double-stranded RNA through the DNA-binding interface and regulated the expression levels of many mRNAs. Furthermore, STK19 knockdown cells exhibited very slow cell proliferation, which cannot be rescued by dimerization or DNA-binding mutants. Therefore, this work concludes that STK19 is highly unlikely to be a kinase but a DNA/RNA-binding protein critical for DNA damage repair (DDR) and cell proliferation. To prevent further confusions, we renamed this protein as TWH19 (Tandem Winged Helix protein formerly known as STK19).

PubMed: 38252411
DOI: 10.1083/jcb.202301090

実験手法

X-RAY DIFFRACTION (1.65 Å)