7XDI

Tail structure of bacteriophage SSV19

Summary for 7XDI

• Entry DOI: 10.2210/pdb7xdi/pdb
• EMDB information: 33148
• Descriptor: VP1, C131, B210, ... (4 entities in total)
• Functional Keywords: complex, viral protein
• Biological source: Sulfolobus spindle-shaped virus; More
• Total number of polymer chains: 6
• Total formula weight: 202868.07

Authors

Liu, H.R.,Chen, W.Y. (deposition date: 2022-03-27, release date: 2022-08-10, Last modification date: 2024-06-26)

Primary citation

Han, Z.,Yuan, W.,Xiao, H.,Wang, L.,Zhang, J.,Peng, Y.,Cheng, L.,Liu, H.,Huang, L.
Structural insights into a spindle-shaped archaeal virus with a sevenfold symmetrical tail.
Proc.Natl.Acad.Sci.USA, 119:e2119439119-e2119439119, 2022

PubMed Abstract: Archaeal viruses with a spindle-shaped virion are abundant and widespread in extremely diverse environments. However, efforts to obtain the high-resolution structure of a spindle-shaped virus have been unsuccessful. Here, we present the structure of SSV19, a spindle-shaped virus infecting the hyperthermophilic archaeon sp. E11-6. Our near-atomic structure reveals an unusual sevenfold symmetrical virus tail consisting of the tailspike, nozzle, and adaptor proteins. The spindle-shaped capsid shell is formed by seven left-handed helical strands, constructed of the hydrophobic major capsid protein, emanating from the highly glycosylated tail assembly. Sliding between adjacent strands is responsible for the variation of a virion in size. Ultrathin sections of the SSV19-infected cells show that SSV19 virions adsorb to the host cell membrane through the tail after penetrating the S-layer. The tailspike harbors a putative endo-mannanase domain, which shares structural similarity to a endo-mannanase. Molecules of glycerol dibiphytanyl glycerol tetraether lipid were observed in hydrophobic clefts between the tail and the capsid shell. The nozzle protein resembles the stem and clip domains of the portals of herpesviruses and bacteriophages, implying an evolutionary relationship among the archaeal, bacterial, and eukaryotic viruses.

PubMed: 35895681
DOI: 10.1073/pnas.2119439119

Experimental method

ELECTRON MICROSCOPY (3.8 Å)