7X7Q

CryoEM structure of RuvA-RuvB-Holliday junction complex

これはPDB形式変換不可エントリーです。

7X7Q の概要

• エントリーDOI: 10.2210/pdb7x7q/pdb
• EMDBエントリー: 33044
• 分子名称: Holliday junction ATP-dependent DNA helicase RuvA, Holliday junction ATP-dependent DNA helicase RuvB, DNA (40-MER), ... (6 entities in total)
• 機能のキーワード: holliday junction, homologous recombination, dna damage repair, branch migration, recombination-dna complex, recombination/dna
• 由来する生物種: Pseudomonas aeruginosa PAO1; 詳細
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 372395.61

構造登録者

Lin, Z.,Qu, Q.,Zhang, X.,Zhou, Z. (登録日: 2022-03-10, 公開日: 2023-03-15, 最終更新日: 2023-09-20)

主引用文献

Zhang, X.,Zhou, Z.,Dai, L.,Chao, Y.,Liu, Z.,Huang, M.,Qu, Q.,Lin, Z.
Cryo-EM structure of the RuvAB-Holliday junction intermediate complex from Pseudomonas aeruginosa.
Front Plant Sci, 14:1139106-1139106, 2023

PubMed Abstract: Holliday junction (HJ) is a four-way structured DNA intermediate in homologous recombination. In bacteria, the HJ-specific binding protein RuvA and the motor protein RuvB together form the RuvAB complex to catalyze HJ branch migration. (, Pa) is a ubiquitous opportunistic bacterial pathogen that can cause serious infection in a variety of host species, including vertebrate animals, insects and plants. Here, we describe the cryo-Electron Microscopy (cryo-EM) structure of the RuvAB-HJ intermediate complex from . The structure shows that two RuvA tetramers sandwich HJ at the junction center and disrupt base pairs at the branch points of RuvB-free HJ arms. Eight RuvB subunits are recruited by the RuvA octameric core and form two open-rings to encircle two opposite HJ arms. Each RuvB subunit individually binds a RuvA domain III. The four RuvB subunits within the ring display distinct subdomain conformations, and two of them engage the central DNA duplex at both strands with their C-terminal β-hairpins. Together with the biochemical analyses, our structure implicates a potential mechanism of RuvB motor assembly onto HJ DNA.

PubMed: 37025142
DOI: 10.3389/fpls.2023.1139106

実験手法

ELECTRON MICROSCOPY (7.02 Å)