7X1H

Cryo-EM structure of human BTR1 in the inward-facing state with R125H mutation

Summary for 7X1H

• Entry DOI: 10.2210/pdb7x1h/pdb
• EMDB information: 32941
• Descriptor: Isoform 1 of Solute carrier family 4 member 11 (1 entity in total)
• Functional Keywords: slc4a11, btr1, slc transporter, membrane protein
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 2
• Total formula weight: 199368.88

Authors

Yin, Y.,Lu, Y.,Zuo, P. (deposition date: 2022-02-24, release date: 2023-10-04, Last modification date: 2023-10-11)

Primary citation

Lu, Y.,Zuo, P.,Chen, H.,Shan, H.,Wang, W.,Dai, Z.,Xu, H.,Chen, Y.,Liang, L.,Ding, D.,Jin, Y.,Yin, Y.
Structural insights into the conformational changes of BTR1/SLC4A11 in complex with PIP 2.
Nat Commun, 14:6157-6157, 2023

PubMed Abstract: BTR1 (SLC4A11) is a NH stimulated H (OH) transporter belonging to the SLC4 family. Dysfunction of BTR1 leads to diseases such as congenital hereditary endothelial dystrophy (CHED) and Fuchs endothelial corneal dystrophy (FECD). However, the mechanistic basis of BTR1 activation by alkaline pH, transport activity regulation and pathogenic mutations remains elusive. Here, we present cryo-EM structures of human BTR1 in the outward-facing state in complex with its activating ligands PIP and the inward-facing state with the pathogenic R125H mutation. We reveal that PIP binds at the interface between the transmembrane domain and the N-terminal cytosolic domain of BTR1. Disruption of either the PIP binding site or protonation of PIP phosphate groups by acidic pH can transform BTR1 into an inward-facing conformation. Our results provide insights into the mechanisms of how the transport activity and conformation changes of BTR1 are regulated by PIP binding and interaction of TMD and NTD.

PubMed: 37788993
DOI: 10.1038/s41467-023-41924-0

Experimental method

ELECTRON MICROSCOPY (2.96 Å)