7WZC

An open conformation Form2 of switch II for RhoA GDP-bound state

Summary for 7WZC

• Entry DOI: 10.2210/pdb7wzc/pdb
• Descriptor: Transforming protein RhoA, GLYCEROL, GUANOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• Functional Keywords: small gtpase, rho family member, cell invasion
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 21496.46

Authors

Jiang, H.,Luo, C. (deposition date: 2022-02-17, release date: 2023-02-22, Last modification date: 2023-11-29)

Primary citation

Jiang, H.,Zu, S.,Lu, Y.,Sun, Z.,Adeerjiang, A.,Guo, Q.,Zhang, H.,Dong, C.,Wu, Q.,Ding, H.,Du, D.,Wang, M.,Liu, C.,Tang, Y.,Liang, Z.,Luo, C.
A RhoA structure with switch II flipped outward revealed the conformational dynamics of switch II region.
J.Struct.Biol., 215:107942-107942, 2023

PubMed Abstract: Small GTPase RhoA switches from GTP-bound state to GDP-bound state by hydrolyzing GTP, which is accelerated by GTPases activating proteins (GAPs). However, less study of RhoA structural dynamic changes was conducted during this process, which is essential for understanding the molecular mechanism of GAP dissociation. Here, we solved a RhoA structure in GDP-bound state with switch II flipped outward. Because lacking the intermolecular interactions with guanine nucleotide, we proposed this conformation of RhoA could be an intermediate after GAP dissociation. Further molecular dynamics simulations found the conformational changes of switch regions are indeed existing in RhoA and involved in the regulation of GAP dissociation and GEF recognition. Besides, the guanine nucleotide binding pocket extended to switch II region, indicating a potential "druggable" cavity for RhoA. Taken together, our study provides a deeper understanding of the dynamic properties of RhoA switch regions and highlights the direction for future drug development.

PubMed: 36781028
DOI: 10.1016/j.jsb.2023.107942

Experimental method

X-RAY DIFFRACTION (1.7994492129 Å)