7WTM
Cryo-EM structure of a yeast pre-40S ribosomal subunit - State Dis-E
This is a non-PDB format compatible entry.
Summary for 7WTM
| Entry DOI | 10.2210/pdb7wtm/pdb |
| EMDB information | 32791 |
| Descriptor | 18S rRNA, 40S ribosomal protein S14-A, 40S ribosomal protein S22-A, ... (18 entities in total) |
| Functional Keywords | ribosome biogenesis, 40s ribosome, ribosome |
| Biological source | Saccharomyces cerevisiae More |
| Total number of polymer chains | 17 |
| Total formula weight | 909744.27 |
| Authors | Cheng, J.,La Venuta, G.,Lau, B.,Berninghausen, O.,Beckmann, R.,Hurt, E. (deposition date: 2022-02-05, release date: 2022-10-19, Last modification date: 2024-06-26) |
| Primary citation | Cheng, J.,La Venuta, G.,Lau, B.,Berninghausen, O.,Beckmann, R.,Hurt, E. In vitro structural maturation of an early stage pre-40S particle coupled with U3 snoRNA release and central pseudoknot formation. Nucleic Acids Res., 50:11916-11923, 2022 Cited by PubMed Abstract: The transition of the 90S to the pre-40S pre-ribosome is a decisive step in eukaryotic small subunit biogenesis leading to a first pre-40S intermediate (state Dis-C or primordial pre-40S), where the U3 snoRNA keeps the nascent 18S rRNA locally immature. We in vitro reconstitute the ATP-dependent U3 release from this particle, catalyzed by the helicase Dhr1, and follow this process by cryo-EM revealing two successive pre-40S intermediates, Dis-D and Dis-E. The latter has lost not only U3 but all residual 90S factors including the GTPase Bms1. In vitro remodeling likewise induced the formation of the central pseudoknot, a universally conserved tertiary RNA structure that comprises the core of the small subunit decoding center. Thus, we could structurally reveal a key tertiary RNA folding step that is essential to form the active 40S subunit. PubMed: 36263816DOI: 10.1093/nar/gkac910 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.5 Å) |
Structure validation
Download full validation report
