7WM3

hnRNP A2/B1 RRMs in complex with single-stranded DNA

Summary for 7WM3

• Entry DOI: 10.2210/pdb7wm3/pdb
• Descriptor: Heterogeneous nuclear ribonucleoproteins A2/B1, DNA (5'-D(P*TP*TP*AP*GP*GP*GP*TP*TP*AP*GP*GP*G)-3') (3 entities in total)
• Functional Keywords: homodimerization, u-shaped structured dna, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 8
• Total formula weight: 97230.54

Authors

Abula, A.,Liu, Y.,Guo, H.,Li, T.,Ji, X. (deposition date: 2022-01-14, release date: 2022-06-29, Last modification date: 2023-11-29)

Primary citation

Liu, Y.,Abula, A.,Xiao, H.,Guo, H.,Li, T.,Zheng, L.,Chen, B.,Nguyen, H.C.,Ji, X.
Structural Insight Into hnRNP A2/B1 Homodimerization and DNA Recognition.
J.Mol.Biol., 435:167920-167920, 2022

PubMed Abstract: Heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1) has been identified as a nuclear DNA sensor. Upon viral infection, hnRNP A2/B1 recognizes pathogen-derived DNA as a homodimer, which is a prerequisite for its translocation to the cytoplasm to activate the interferon response. However, the DNA binding mechanism inducing hnRNP A2/B1 homodimerization is unknown. Here, we show the crystal structure of the RNA recognition motif (RRM) of hnRNP A2/B1 in complex with a U-shaped ssDNA, which mediates the formation of a newly observed protein dimer. Our biochemical assays and mutagenesis studies confirm that the hnRNP A2/B1 homodimer forms in solution by binding to pre-generated ssDNA or dsDNA with a U-shaped bulge. These results depict a potential functional state of hnRNP A2/B1 in antiviral immunity and other cellular processes.

PubMed: 36528084
DOI: 10.1016/j.jmb.2022.167920

Experimental method

X-RAY DIFFRACTION (1.62 Å)