7WBP

Crystal structure of the receptor binding domain of SARS-CoV-2 Omicron variant spike glycoprotein in complex with its receptor human ACE2

7WBP の概要

• エントリーDOI: 10.2210/pdb7wbp/pdb
• 分子名称: Angiotensin-converting enzyme 2, Spike protein S1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: complex, viral protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 96050.31

構造登録者

Qi, J.,Han, P. (登録日: 2021-12-17, 公開日: 2022-01-19, 最終更新日: 2024-10-30)

主引用文献

Han, P.,Li, L.,Liu, S.,Wang, Q.,Zhang, D.,Xu, Z.,Han, P.,Li, X.,Peng, Q.,Su, C.,Huang, B.,Li, D.,Zhang, R.,Tian, M.,Fu, L.,Gao, Y.,Zhao, X.,Liu, K.,Qi, J.,Gao, G.F.,Wang, P.
Receptor binding and complex structures of human ACE2 to spike RBD from omicron and delta SARS-CoV-2.
Cell, 185:630-640.e10, 2022

PubMed Abstract: The coronavirus disease 2019 (COVID-19) pandemic continues worldwide with many variants arising, some of which are variants of concern (VOCs). A recent VOC, omicron (B.1.1.529), which obtains a large number of mutations in the receptor-binding domain (RBD) of the spike protein, has risen to intense scientific and public attention. Here, we studied the binding properties between the human receptor ACE2 (hACE2) and the VOC RBDs and resolved the crystal and cryoelectron microscopy structures of the omicron RBD-hACE2 complex as well as the crystal structure of the delta RBD-hACE2 complex. We found that, unlike alpha, beta, and gamma, omicron RBD binds to hACE2 at a similar affinity to that of the prototype RBD, which might be due to compensation of multiple mutations for both immune escape and transmissibility. The complex structures of omicron RBD-hACE2 and delta RBD-hACE2 reveal the structural basis of how RBD-specific mutations bind to hACE2.

PubMed: 35093192
DOI: 10.1016/j.cell.2022.01.001

実験手法

X-RAY DIFFRACTION (3 Å)