7WB1

PlmCasX-sgRNAv2-dsDNA ternary complex at nts loading state

Summary for 7WB1

• Entry DOI: 10.2210/pdb7wb1/pdb
• EMDB information: 32392
• Descriptor: TS-DNA, NTS-DNA, RNA (121-MER), ... (4 entities in total)
• Functional Keywords: crispr, casx, sgrna, r-loop complex, rna binding protein, dna binding protein, rna binding protein-rna-dna complex, rna binding protein/rna/dna
• Biological source: Planctomycetes bacterium; More
• Total number of polymer chains: 4
• Total formula weight: 176057.45

Authors

Zhang, S.,Liu, J.J.G. (deposition date: 2021-12-15, release date: 2022-03-16, Last modification date: 2024-06-26)

• Primary citation: Tsuchida, C.A.,Zhang, S.,Doost, M.S.,Zhao, Y.,Wang, J.,O'Brien, E.,Fang, H.,Li, C.P.,Li, D.,Hai, Z.Y.,Chuck, J.,Brotzmann, J.,Vartoumian, A.,Burstein, D.,Chen, X.W.,Nogales, E.,Doudna, J.A.,Liu, J.G.; Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity.; Mol.Cell, 82:1199-1209.e6, 2022; PubMed: 35219382; DOI: 10.1016/j.molcel.2022.02.002

Experimental method

ELECTRON MICROSCOPY (3.7 Å)